StressCovidV1, Main, Exploration, bibRecord, 000A65

SP-LL-37, human antimicrobial peptide, enhances disease resistance in transgenic rice.

Identifieur interne : 000A65 ( Main/Exploration ); précédent : 000A64; suivant : 000A66

SP-LL-37, human antimicrobial peptide, enhances disease resistance in transgenic rice.

Auteurs : In Hye Lee [Corée du Sud] ; Yu-Jin Jung [Corée du Sud] ; Yong Gu Cho [Corée du Sud] ; Ill Sup Nou [Corée du Sud] ; Md Amdadul Huq [Corée du Sud] ; Franz Marielle Nogoy [Corée du Sud] ; Kwon-Kyoo Kang [Corée du Sud]

Source :

PloS one [ 1932-6203 ] ; 2017.

RBID : pubmed:28282452

Descripteurs français

English descriptors

KwdEn :
- Agrobacterium (genetics), Anti-Infective Agents (metabolism), Anti-Infective Agents (pharmacology), Antimicrobial Cationic Peptides (analysis), Antimicrobial Cationic Peptides (genetics), Antimicrobial Cationic Peptides (metabolism), Base Sequence, DNA (genetics), DNA (metabolism), Disease Resistance (drug effects), Enzyme-Linked Immunosorbent Assay, Genetic Vectors (genetics), Genetic Vectors (metabolism), Humans, Microscopy, Fluorescence, Molecular Sequence Data, Oryza (metabolism), Plant Diseases (prevention & control), Plants, Genetically Modified (metabolism), Tobacco (metabolism).
MESH :
- chemical , analysis : Antimicrobial Cationic Peptides.
- chemical , genetics : Antimicrobial Cationic Peptides, DNA.
- chemical , metabolism : Anti-Infective Agents, Antimicrobial Cationic Peptides, DNA.
- drug effects : Disease Resistance.
- genetics : Agrobacterium, Genetic Vectors.
- metabolism : Genetic Vectors, Oryza, Plants, Genetically Modified, Tobacco.
- chemical , pharmacology : Anti-Infective Agents.
- prevention & control : Plant Diseases.
- Base Sequence, Enzyme-Linked Immunosorbent Assay, Humans, Microscopy, Fluorescence, Molecular Sequence Data.

Abstract

Human LL-37 is a multifunctional antimicrobial peptide of cathelicidin family. It has been shown in recent studies that it can serve as a host's defense against influenza A virus. We now demonstrate in this study how signal peptide LL-37 (SP-LL-37) can be used in rice resistance against bacterial leaf blight and blast. We synthesized LL-37 peptide and subcloned in a recombinant pPZP vector with pGD1 as promoter. SP-LL-37 was introduced into rice plants by Agrobacterium mediated transformation. Stable expression of SP-LL-37 in transgenic rice plants was confirmed by RT-PCR and ELISA analyses. Subcellular localization of SP-LL-37-GFP fusion protein showed evidently in intercellular space. Our data on testing for resistance to bacterial leaf blight and blast revealed that the transgenic lines are highly resistant compared to its wildtype. Our results suggest that LL-37 can be further explored to improve wide-spectrum resistance to biotic stress in rice.

DOI: 10.1371/journal.pone.0172936
PubMed: 28282452

Affiliations:

Corée du Sud

Le document en format XML

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<author><name sortKey="Lee, In Hye" sort="Lee, In Hye" uniqKey="Lee I" first="In Hye" last="Lee">In Hye Lee</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Horticultural Life Science, Hankyong National University, Ansung, Korea.</nlm:affiliation>
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<author><name sortKey="Jung, Yu Jin" sort="Jung, Yu Jin" uniqKey="Jung Y" first="Yu-Jin" last="Jung">Yu-Jin Jung</name>
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<author><name sortKey="Cho, Yong Gu" sort="Cho, Yong Gu" uniqKey="Cho Y" first="Yong Gu" last="Cho">Yong Gu Cho</name>
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<term>Enzyme-Linked Immunosorbent Assay</term>
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<term>Genetic Vectors (metabolism)</term>
<term>Humans</term>
<term>Microscopy, Fluorescence</term>
<term>Molecular Sequence Data</term>
<term>Oryza (metabolism)</term>
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<term>Plants, Genetically Modified (metabolism)</term>
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<term>Anti-infectieux (pharmacologie)</term>
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<term>Maladies des plantes ()</term>
<term>Microscopie de fluorescence</term>
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<term>Peptides antimicrobiens cationiques (génétique)</term>
<term>Peptides antimicrobiens cationiques (métabolisme)</term>
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<term>Séquence nucléotidique</term>
<term>Tabac (métabolisme)</term>
<term>Test ELISA</term>
<term>Vecteurs génétiques (génétique)</term>
<term>Vecteurs génétiques (métabolisme)</term>
<term>Végétaux génétiquement modifiés (métabolisme)</term>
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</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antimicrobial Cationic Peptides</term>
<term>DNA</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Anti-Infective Agents</term>
<term>Antimicrobial Cationic Peptides</term>
<term>DNA</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Peptides antimicrobiens cationiques</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Disease Resistance</term>
</keywords>
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</keywords>
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<term>Peptides antimicrobiens cationiques</term>
<term>Vecteurs génétiques</term>
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<term>Oryza</term>
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<term>Tobacco</term>
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<term>Oryza</term>
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<front><div type="abstract" xml:lang="en">Human LL-37 is a multifunctional antimicrobial peptide of cathelicidin family. It has been shown in recent studies that it can serve as a host's defense against influenza A virus. We now demonstrate in this study how signal peptide LL-37 (SP-LL-37) can be used in rice resistance against bacterial leaf blight and blast. We synthesized LL-37 peptide and subcloned in a recombinant pPZP vector with pGD1 as promoter. SP-LL-37 was introduced into rice plants by Agrobacterium mediated transformation. Stable expression of SP-LL-37 in transgenic rice plants was confirmed by RT-PCR and ELISA analyses. Subcellular localization of SP-LL-37-GFP fusion protein showed evidently in intercellular space. Our data on testing for resistance to bacterial leaf blight and blast revealed that the transgenic lines are highly resistant compared to its wildtype. Our results suggest that LL-37 can be further explored to improve wide-spectrum resistance to biotic stress in rice.</div>
</front>
</TEI>
<affiliations><list><country><li>Corée du Sud</li>
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SP-LL-37, human antimicrobial peptide, enhances disease resistance in transgenic rice.

SP-LL-37, human antimicrobial peptide, enhances disease resistance in transgenic rice.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

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